{"id":653,"date":"2020-07-17T11:39:07","date_gmt":"2020-07-17T11:39:07","guid":{"rendered":"https:\/\/www.thaihealth.net\/h\/?post_type=encyclopedia&#038;p=653"},"modified":"2020-07-17T11:39:07","modified_gmt":"2020-07-17T11:39:07","slug":"the-d614g-mutation-of-sars-cov-2-spike-protein-enhances-viral-infectivity-and-decreases-neutralization-sensitivity-to-individual-convalescent-sera","status":"publish","type":"encyclopedia","link":"https:\/\/thaihealth.net\/h\/encyclopedia\/the-d614g-mutation-of-sars-cov-2-spike-protein-enhances-viral-infectivity-and-decreases-neutralization-sensitivity-to-individual-convalescent-sera","title":{"rendered":"The D614G mutation of SARS-CoV-2 spike protein enhances viral infectivity and decreases neutralization sensitivity to individual convalescent sera"},"content":{"rendered":"<p><strong>The D614G mutation of SARS-CoV-2 spike protein enhances viral infectivity and decreases neutralization sensitivity to individual convalescent sera<\/strong>Jie Hu, Chang-Long He, Qing-Zhu Gao, Gui-Ji Zhang, Xiao-Xia Cao, Quan-Xin Long, Hai-Jun Deng, Lu-Yi Huang, Juan Chen,  View ORCID ProfileKai Wang, Ni Tang, Ai-Long Huang<br>\ndoi: https:\/\/doi.org\/10.1101\/2020.06.20.161323<br>\n<strong>Abstract<\/strong><br>\nCoronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The spike protein that mediates SARS-CoV-2 entry into host cells, is one of the major targets for vaccines and therapeutics. Thus, insights into the sequence variations of S protein are key to understanding the infection and antigenicity of SARS-CoV-2. Here, we observed a dominant mutational variant at the 614 position of S protein (aspartate to glycine, D614G mutation). Using pseudovirus-based assay, we found that S-D614 and S-G614 protein pseudotyped viruses share a common receptor, human angiotensin-converting enzyme 2 (ACE2), which could be blocked by recombinant ACE2 with the fused Fc region of human IgG1. However, S-D614 and S-G614 protein demonstrated functional differences. First, S-G614 protein could be cleaved by serine protease elastase-2 more efficiently. Second, S-G614 pseudovirus infected 293T-ACE2 cells significantly more efficiently than the S-D614 pseudovirus, Moreover, 93% (38\/41) sera from convalescent COVID-19 patients could neutralize both S-D614 and S-G614 pseudotyped viruses with comparable efficiencies, but about 7% (3\/41) convalescent sera showed decreased neutralizing activity against S-G614 pseudovirus. These findings have important implications for SARS-CoV-2 transmission and immune interventions.<\/p>\n<p>Competing Interest Statement<br>\nThe authors have declared no competing interest.<br>\nFull paper here<br>\nhttps:\/\/www.biorxiv.org\/content\/10.1101\/2020.06.20.161323v1<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The D614G mutation of SARS-CoV-2 spike protein enhances viral infectivity and decreases neutralization sensitivity to individual convalescent seraJie Hu, Chang-Long He, Qing-Zhu Gao, Gui-Ji Zhang,&hellip;<\/p>\n","protected":false},"author":1,"template":"","meta":[],"encyclopedia-tag":[],"class_list":["post-653","encyclopedia","type-encyclopedia","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/encyclopedia\/653","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/encyclopedia"}],"about":[{"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/types\/encyclopedia"}],"author":[{"embeddable":true,"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/users\/1"}],"wp:attachment":[{"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/media?parent=653"}],"wp:term":[{"taxonomy":"encyclopedia-tag","embeddable":true,"href":"https:\/\/thaihealth.net\/h\/wp-json\/wp\/v2\/encyclopedia-tag?post=653"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}